upci scc090 cells  (DSMZ)

Journal: Radiology and Oncology

Article Title: Human papillomavirus-related oropharyngeal squamous cell carcinoma exhibits enhanced radiosensitivity despite limited activation of cytosolic DNA sensing pathways and innate immune responses

doi: 10.2478/raon-2025-0057

Figure Lengend Snippet: The effect of irradiation (IR) on cell survival and tumor growth. (A) Survival of cells after in vitro IR with 2, 4, 6, 8, and 10 Gy (n = 3). (B) Kaplan-Meier survival curve for mice bearing pharyngeal squamous cell carcinoma (PSCC) tumors treated with either a single dose of 8 Gy or 3×8 Gy, complete response (CR) (n = 6). Data are presented as mean ± standard error of the mean (SEM); # = indicates p < 0.05 for comparisons between UPCI:SCC090 and other cell lines or tumor models; * = indicates p < 0.05 for comparisons between IR doses within the same cell line or tumor model.

Article Snippet: The in vitro experiments were performed with four human PSCC cell lines: HPV16-related OPSCC UPCI:SCC090 (RRID:CVCL_1899; ATCC ® CRL-3239TM, Manassas, VA, USA), HPV16-unrelated OPSCC UM-SCC-6 (RRID:CVCL_7773; Merck-Millipore, Burlington, MA, USA), HPV16-related hypopharyngeal squamous cell carcinoma (HPSCC) 2A3 (RRID:CVCL_0D71; ATCC ® CRL-3212TM, ATCC), and HPV16-unrelated HPSCC FaDu (RRID:CVCL_1218; ATCC ® HTB-43TM, ATCC).

Techniques: Irradiation, In Vitro

Journal: Radiology and Oncology

Article Title: Human papillomavirus-related oropharyngeal squamous cell carcinoma exhibits enhanced radiosensitivity despite limited activation of cytosolic DNA sensing pathways and innate immune responses

doi: 10.2478/raon-2025-0057

Figure Lengend Snippet: Irradiation (IR)-induced accumulation of dsDNA in the cytosol of pharyngeal squamous cell carcinoma (PSCC) cells. (A) Accumulation of dsDNA in the cytosol of cells 72 hours after IR. Green: dsDNA (anti-dsDNA), red: plasma membrane (WGA), blue: nucleus (Hoechst 33342), Scale bar = 10 μm. (B) Number of dsDNA spots per cell in cytosol 72 hours after IR with 4, 8, or 3x8 Gy (n = 8). Data is represented as mean ± standard error of the mean (SEM). # = indicates p < 0.05 for comparisons between UPCI:SCC090 and other cell lines or tumor models; * = indicates p < 0.05 for comparisons between IR doses within the same cell line or tumor model; o = indicates p < 0.05 for comparisons between FaDu and 2A3 models; + = indicates p < 0.05 for comparisons between UM-SCC-6 and FaDu

Article Snippet: The in vitro experiments were performed with four human PSCC cell lines: HPV16-related OPSCC UPCI:SCC090 (RRID:CVCL_1899; ATCC ® CRL-3239TM, Manassas, VA, USA), HPV16-unrelated OPSCC UM-SCC-6 (RRID:CVCL_7773; Merck-Millipore, Burlington, MA, USA), HPV16-related hypopharyngeal squamous cell carcinoma (HPSCC) 2A3 (RRID:CVCL_0D71; ATCC ® CRL-3212TM, ATCC), and HPV16-unrelated HPSCC FaDu (RRID:CVCL_1218; ATCC ® HTB-43TM, ATCC).

Techniques: Irradiation, Clinical Proteomics, Membrane

Journal: Radiology and Oncology

Article Title: Human papillomavirus-related oropharyngeal squamous cell carcinoma exhibits enhanced radiosensitivity despite limited activation of cytosolic DNA sensing pathways and innate immune responses

doi: 10.2478/raon-2025-0057

Figure Lengend Snippet: Effect of irradiation (IR) on cytokine gene expression in tumor cells and tumor microenvironment (TME) of pharyngeal squamous cell carcinoma (PSCC). (A–C) Fold change in expression of IFNβ (A) , tumor necrosis factor (TNF)α (B) , and IL1β (C) in vitro 72 hours after IR with 4, 8, or 3x8 Gy, normalized to housekeeping genes (GUSB and B2M) and respective controls (n = 3). (D–F) Fold change in expression of IFNβ (D) , TNFa (E) , and IL1 β (F) in tumor cells in vivo 72 hours after IR with 8 or 3×8 Gy, normalized to housekeeping genes (GUSB and B2M) and control (n = 5). (G–I) Fold change in expression of Ifnβ (G) , TNFα (H) , and Il1β (I) in the TME in vivo 72 hours after IR with 8 or 3–8 Gy, normalized to housekeeping genes (BA and GADPH) and control (n = 5). Data is represented as mean ± standard error of the mean (SEM). # = indicates p < 0.05 for comparisons between UPCI:SCC090 and other cell lines or tumor models; * = indicates p < 0.05 for comparisons between IR doses within the same cell line or tumor model; o = indicates p < 0.05 for comparisons between FaDu and 2A3 models; + = indicates p < 0.05 for comparisons between UM-SCC-6 and FaDu

Article Snippet: The in vitro experiments were performed with four human PSCC cell lines: HPV16-related OPSCC UPCI:SCC090 (RRID:CVCL_1899; ATCC ® CRL-3239TM, Manassas, VA, USA), HPV16-unrelated OPSCC UM-SCC-6 (RRID:CVCL_7773; Merck-Millipore, Burlington, MA, USA), HPV16-related hypopharyngeal squamous cell carcinoma (HPSCC) 2A3 (RRID:CVCL_0D71; ATCC ® CRL-3212TM, ATCC), and HPV16-unrelated HPSCC FaDu (RRID:CVCL_1218; ATCC ® HTB-43TM, ATCC).

Techniques: Irradiation, Gene Expression, Expressing, In Vitro, In Vivo, Control

Journal: Cell reports

Article Title: Lidocaine induces apoptosis in head and neck squamous cell carcinoma through activation of bitter taste receptor T2R14

doi: 10.1016/j.celrep.2023.113437

Figure Lengend Snippet: HNSSC cell lines SCC47, FaDu, RPMI2650, SCC4, and SCC90 were imaged for Ca2+ responses with bitter agonists. (A and B) SCC47 Ca2+ response over time (A) and peak Ca2+ responses (B) with lidocaine; mean ± SEM with >3 experiments using separate cultures. Significance by one-way ANOVA with Bonferroni post-test comparing HBSS with each concentration of lidocaine. (C and D) SCC47 Ca2+ response over time (C) and peak Ca2+ responses (D) with denatonium benzoate; mean ± SEM with >3 experiments using separate cultures. Significance by one-way ANOVA with Bonferroni post-test comparing HBSS to each concentration of denatonium. (E) Ca2+ dose response of lidocaine in SCC47 cells. (F–H) SCC47 (F), FaDu (G), and RPMI2650 (H) peak Ca2+ responses with HBSS, lidocaine, denatonium benzoate, thujone, flufenamic acid (FFA), or ATP. Mean ± SEM with >3 experiments using separate cultures. Significance by one-way ANOVA with Bonferroni post-test comparing HBSS to each agonist. (I–K) SCC4 (I), SCC90 (J), and FaDu (K) peak fluorescent Ca2+ responses with lidocaine; mean ± SEM with >3 experiments using separate cultures. Significance by one-way ANOVA with Bonferroni post-test comparing HBSS to each concentration of lidocaine. (L) SCC47 peak Ca2+ response with lidocaine or procaine; mean ± SEM with >3 experiments using separate cultures. Significance by unpaired t test. *p < 0.05, **p < 0.01, ***p < 0.001; ns, no statistical significance.

Article Snippet: SCC90 , ATCC , CRL-3239.

Techniques: Concentration Assay

Journal: Cell reports

Article Title: Lidocaine induces apoptosis in head and neck squamous cell carcinoma through activation of bitter taste receptor T2R14

doi: 10.1016/j.celrep.2023.113437

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: SCC90 , ATCC , CRL-3239.

Techniques: Virus, Recombinant, Reverse Transcription, DC Protein Assay, Western Blot, CRISPR, Control, Expressing, TaqMan Assay, shRNA, Software